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KMID : 1234420100380040442
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 4 p.442 ~ p.447
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Analysis of Differentially Expressed Genes by Allicin in Human Colorectal Cancer Cell.
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Kim Kyung-Ho
Kim Min-Jeong
Kim Jong-Sik
Pyo Suhk-Neung
Kim Byung-Oh
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Abstract
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We investigated anti-cancer and anti-proliferative activity of allicin and analyzed global gene expression changes by allicin treatment in human colorectal HCT116 cells. As a result, allicin decreased cell viabilities in a dose and time-dependent manner and induced apoptosis. Oligo DNA microarray analysis, we found that 7,840 genes were up-regulated more than 2-folds, whereas 10,010 genes were down-regulated more than 2-folds by 50 μM allicin treatment. To confirm specific gene expressions, we performed RT-PCR. Consistent with the results of DNA microarray analysis, allicin dramatically induced ATF3 and NAG1 gene expression. Interestingly, NAG-1 protein expression was dependent on p53 presence. Taken together, our present results increase the knowledge of the molecular mechanism of anti-cancer and anti-proliferative activity mediated by allciin in human colorectal cancer cell.
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KEYWORD
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Allicin, oligo DNA microarray, gene expression, ATF3, colorectal cancer
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